The goal of this hands-on course is to teach the fundamentals of RNA and DNA library preparation for next generation sequencing applications. RNA depletion and enrichment strategies for different populations of RNA will be explained and users will learn about the advantages of RNA-Seq compared to other methods such as Sanger sequencing and microarrays.
In addition, library preparation for (low input) DNA samples will be performed. Users will also learn about sample QC and library quantification that enable the preparation of high-quality, low-biased DNA libraries.
Participants are highly encouraged to bring their own RNA and DNA samples to this hands-on course.
The bioinformatics part of the course will cover tools for RNA-seq data analysis using open-source software. Computers for hands-on exercises will be provided along with demo data sets.
Date: 1 – 4 July 2019
Location: Heidelberg, Germany