Single molecule microRNA technology proves useful for liver toxicity detection
Breakthrough research published in PLOS ONE demonstrates quantitative, PCR-free detection of microRNA
Quanterix Corporation, a company digitizing biomarker analysis with the goal of advancing the science of precision health, and DestiNA Genomics Ltd, a manufacturer of patented reagents for nucleic acid detection, today announced that PLOS ONE has published a proof-of-concept single probe method for detecting microRNA biomarkers associated with liver toxicity.
Published jointly by Quanterix, DestiNA Genomics Ltd., Edinburgh University and the Pfizer-Universidad de Granada-Junta de Andalucía Centre for Genomics and Oncological Research (GENyO), the study, titled, “Polymerase-free measurement of microRNA-122 with single base specificity using single molecule arrays: Detection of drug-induced liver injury,” details a clinically meaningful method for detecting drug-induced liver injury using the Single Molecule Array Detection technology, or Simoa™, developed by Quanterix, in association with the ‘SMART Nucleobase’ and probe chemistry by DestiNA.
“Drug-induced liver damage is a major health issue for patients and presents unique testing and treatment challenges for doctors and drug developers,” said Dr. James Dear, Reader in Clinical Pharmacology at the University of Edinburgh and co-author of the paper. “To optimally manage liver damage as a result of medicines, we need to identify biomarkers that can quickly and easily measure drug-induced liver injury. This new method provides an accurate measurement of miR-122 with user-independent sensitivity with a time-to-result that is needed to develop tests that can improve patient safety.”
miRNA-122 (miR-122) is an emerging biomarker shown to provide more sensitive, specific detection of drug-inducted liver toxicity. While it has been proven to be more effective than an alanine transaminase (ALT) enzyme test in analyzing liver toxicity, previous workflow challenges associated with standard PCR-based assays along with the lack of speed and reproducibility needed for routine clinical testing, have stalled the progression of miR-122 in clinical research.
“It’s an exciting breakthrough in RNA detection to learn that microRNA serve as valuable clinical research biomarkers,” said Hugh Ilyine, DestiNA’s CEO. “Using a PCR-free approach that delivers a simpler, faster preparation and analysis time, but with higher accuracy, means easy microRNA detection from serum and plasma is finally possible and opens up the development of microRNA as valuable clinical biomarkers.”
miR-122 was measured in the serum of patients who had overdosed on acetaminophen and sustained clinically significant liver injury. Serum samples were tested for miR-122 using the single molecule assay and quantitative PCR.
The high specificity (>3 × 10^7-fold) in the Simoa assay was achieved from the SMARTbase technology using only a single probe rather than multiple probes and primers used in PCR. The use of a single probe greatly simplified the assay designed while maintaining the ability to discriminate target sequences with single base specificity.
The data indicate that the sensitivity and specificity of the Simoa assay was sufficient to measure miR-122 in all patients, and showed a clear distinction between healthy controls and those with clinical liver toxicity after drug overdose, as was the case for PCR.
“The potential to easily and quantifiably detect nucleic acids using our Simoa assay shows new opportunity for the technology and for researchers studying the clinical implications of RNA,” said David Duffy, Vice President, Research and Chief Technology Officer at Quanterix. “This paper serves as an initial proof-of-concept and encourages our thinking about potential applications using the Simoa technology beyond liver toxicity, including liquid biopsies, early and rapid diagnosis of sepsis and pharmacokinetic measurements of RNA and DNA therapeutics.”
To read the full study published in PLOS ONE, please visit: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0179669
Quanterix is a company that is digitizing biomarker analysis with the goal of advancing the science of precision health. The company’s digital health solution, Simoa, has the potential to change the way in which healthcare is provided today by giving researchers the ability to closely examine the continuum from health to disease. Quanterix’s technology is designed to enable much earlier disease detection, better prognosis and enhanced treatment methods to improve the quality of life and longevity of the population for generations to come. The technology is currently being used for applications in several therapeutic areas, including oncology, neurology, cardiology, inflammation and infectious disease. The company was established in 2007 and is located in Lexington, Massachusetts.
About DestiNA Genomics
Destina Genomics Ltd is a Scottish company with R&D laboratories and a subsidiary in Granada, Spain. Since its start-up as a spin-out from the University of Edinburgh, it has been developing and refining its proprietary ‘SMART Nucleobase and custom probe’ chemistry that enables high specificity and ‘false positive’ free detection of nucleic acids and their mutations, across a range of platforms. The DestiNA reagents make ideal partners for platforms that include Mass Spectrometry, Membrane flow, photonic and sound wave sensors, and the Quanterix ultra-sensitive digital Simoa. DestiNA technology is very well suited for liquid biopsies, and for the detection of nucleic acid biomarkers valuable for detecting cancers, illness and infectious diseases, as well as liver toxicity. It has focused on PCR-free direct detection of microRNAs from plasma and serum, given the current challenges to deliver cost effective and reliable tests for the academic research community, pharma and biotech drug development, and improved clinical decision and treatment monitoring of patients.