Citrus is one of the most important and widely grown fruit crop with global production ranking firstly among all the fruit crops in the world. Sweet orange accounts for more than half of the Citrus production both in fresh fruit ...
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RNA sequencing read depth requirement for optimal transcriptome coverage
One of the concerns of assembling de novo transcriptomes is determining the amount of read sequences required to ensure a comprehensive coverage of genes expressed in a particular sample. In this report, researchers from the Rubber Research Institute of Malaysia ...
Read More »SHEAR: sample heterogeneity estimation and assembly by reference
Personal genome assembly is a critical process when studying tumor genomes and other highly divergent sequences. The accuracy of downstream analyses, such as RNA-seq and ChIP-seq, can be greatly enhanced by using personal genomic sequences rather than standard references. Unfortunately, ...
Read More »NGSANE: A Lightweight Production Informatics Framework for High Throughput Data Analysis
The initial steps in the analysis of Next Generation Sequencing (NGS) data can be automated by way of software ‘pipelines’. However, individual components depreciate rapidly due to evolving technology and analysis methods, often rendering entire versions of production informatics pipelines ...
Read More »HTSstation: A Web Application and Open-Access Libraries for High-Throughput Sequencing Data Analysis
The HTSstation analysis portal is a suite of simple web forms coupled to modular analysis pipelines for various applications of High-Throughput Sequencing including ChIP-seq, RNA-seq, 4C-seq and re-sequencing. HTSstation offers biologists the possibility to rapidly investigate their HTS data using ...
Read More »RNA-seq QC in GenePattern
This tutorial covers common quality control metrics that can be used for evaluating aligned RNA-seq data files in GenePattern
Read More »A comparison of RNA-seq and exon arrays for whole genome transcription profiling
The past decade has seen an abundance of transcriptional profiling studies of preclinical models of persistent pain, predominantly employing microarray technology. In this study a team led by scientists from the University College London directly compare exon microarrays to RNA-seq ...
Read More »The Most Informative Spacing Test Effectively Discovers Biologically Relevant Outliers or Multiple Modes in Expression.
Several outlier and subgroup identification statistics (OASIS) have been proposed to discover transcriptomic features with outliers or multiple modes in expression that are indicative of distinct biological processes or subgroups. Here, researchers from the St. Jude Children’s Research Hospital, Memphis borrow ...
Read More »Notes on: Single-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cells
from mathbionerd by Melissa Wilson Sayres Brief background: We have two copies of each non-sex gene. Each version of the gene is called an allele: one inherited from your genetic mother, one from your genetic father. It is generally thought ...
Read More »Quantum Biosystems demonstrates First Reads using Quantum Single Molecule Sequencing
Results Give Glimpse of Future Low Cost, High-throughput Personal DNA Sequencing Chip New Sequencing Technology Data release, Lays Groundwork for Bacterial and Human Sequencing Quantum Biosystems today announced the release of raw data access to its first reads. The company released ...
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