From Proteintech by Dr. Karolina Szczesna
RNA sequencing (RNA-seq) is a widely used method employing next generation sequencing (NGS) to discover and quantify the abundance of RNA molecules in biological samples.
Why do we do RNA-seq?
RNA-seq has revolutionized approach to transcriptomics, it allows high-throughput being precise and cost-effective analysis of RNAs having advantages over microarrays and expressed sequence tag (EST) sequencing.
RNA-seq technology has many applications in basic and clinical research. It is mainly used for:
- functional annotation of genomes by identifying all coding and non-coding RNAs, splice-sites, isoforms, open reading frames
- quantitative analysis of RNA expression and expression profiling
- detection of single nucleotide polymorphisms
- studying RNA editing – RNA posttranscriptional modifications (PTMs)