Technological advances in the sequencing field support in-depth characterization of the transcriptome. There are now many genome-wide RNA sequencing methods used to investigate specific aspects of gene expression and its regulation, from transcription to RNA processing and translation:
- tag-based methods for studying transcription
- alternative initiation and polyadenylation events
- shotgun methods for detection of alternative splicing
- full-length RNA sequencing for the determination of complete transcript structures
- targeted methods for studying the process of transcription and translation
With the ensemble of technologies available, it is now possible to obtain a comprehensive view on transcriptome complexity and the regulation of transcript diversity.