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Tag Archives: assembly PCR

PrimerView – forward and reverse primer design from multi-sequence datasets

June 3, 2015 Leave a comment 12,425 Views

rna-seq

High-throughput primer design is routinely performed in a wide number of molecular applications including genotyping specimens using traditional PCR techniques as well as assembly PCR, nested PCR, and primer walking experiments. Batch primer design is also required in validation experiments ...

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Recent RNA-SEQ Pubs

Prepubertal exposure to a high dose of cadmium induces hypothalamic injury through transcriptome profiling alteration and neuronal degeneration in female rats.
Computationally reconstructing cotranscriptional RNA folding from experimental data reveals rearrangement of non-native folding intermediates.
Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research.
Genome-Wide Discovery of Natural Variation in Pre-mRNA Splicing and Prioritizing Causal Alternative Splicing to Salt Stress Response in Rice.
NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway.
HAND1 and BARX1 act as transcriptional and anatomic determinants of malignancy in gastrointestinal stromal tumor.

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Poly-A enrichment or rRNA depletion – how library construction influences RNA sequencing data

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    AIR-seq – metabolic RNA labeling for probing RNA dynamics in bacteria

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  • Featured RNA-Seq Job – Computational Biology Scientist, Cancer Immunology

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    Enriching pathogen transcripts from infected samples: a capture based approach to improved host-pathogen RNA-Seq

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