Tag Archives: Bias

Comparison of Computational Methods for Identification of Allele-Specific Expression based on Next Generation Sequencing Data

rna-seq

Allele-specific expression (ASE) studies have wide-ranging implications for genome biology and medicine. Whole transcriptome RNA sequencing (RNA-Seq) has emerged as a genome-wide tool for identifying ASE, but suffers from mapping bias favoring reference alleles. Two categories of methods are adopted ...

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RVboost: RNA-Seq variants prioritization using a boosting method

rna-seq

RNA-Seq has become the method of choice to quantify genes and exons, discover novel transcripts, and detect fusion genes. However, reliable variant identification from RNA-Seq data remains challenging due to the complexities of the transcriptome, the challenges of accurately mapping ...

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Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation

rna-seq

The collborators at the SEQC/MAQC-III Consortium present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference ...

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Bioo Scientific First to Address Small RNA-Seq Bias Concerns with Launch of New Kit

rna-seq

Author: BIOO/Monday, August 11, 2014 Bioo Scientific launched the patent pending NEXTflex™ Small RNA Sequencing Kit v2, which utilizes randomized adapters to significantly reduce ligation bias from library preparation, resulting in the most accurate data available, as illustrated in a ...

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