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Tag Archives: Champalimaud Center for the Unkown

Measuring the Expression of SINE Retroelements with RNA-Seq

February 25, 2016 Leave a comment 3,398 Views

rna-seq

The regulation and control of SINE transcription is likely a key process to preserve the physiology and homeostasis of specific tissues and organs. This implies that the analysisof SINE expression is relevant to understand the mechanisms by which these elements ...

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Recent RNA-SEQ Pubs

Prepubertal exposure to a high dose of cadmium induces hypothalamic injury through transcriptome profiling alteration and neuronal degeneration in female rats.
Computationally reconstructing cotranscriptional RNA folding from experimental data reveals rearrangement of non-native folding intermediates.
Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research.
Genome-Wide Discovery of Natural Variation in Pre-mRNA Splicing and Prioritizing Causal Alternative Splicing to Salt Stress Response in Rice.
NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway.
HAND1 and BARX1 act as transcriptional and anatomic determinants of malignancy in gastrointestinal stromal tumor.

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Poly-A enrichment or rRNA depletion – how library construction influences RNA sequencing data

    5 hours ago
  • rna-seq

    AIR-seq – metabolic RNA labeling for probing RNA dynamics in bacteria

    6 hours ago
  • Featured RNA-Seq Job – Computational Biology Scientist, Cancer Immunology

    1 day ago
  • rna-seq

    Enriching pathogen transcripts from infected samples: a capture based approach to improved host-pathogen RNA-Seq

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    Associate Professor

    2 days ago
  • rna-seq

    Using single-cell RNA sequencing to disentangle the blood system

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