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Tag Archives: cluster

Expression dynamics and role of Hunchback in visual system and brain development

January 24, 2018 Leave a comment 1,744 Views

The development of different cell types must be tightly coordinated, and the eye-antennal imaginal discs of Drosophila melanogaster represent an excellent model to study the molecular mechanisms underlying this coordination. These imaginal discs contain the anlagen of nearly all adult head structures, ...

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Recent RNA-SEQ Pubs

Prepubertal exposure to a high dose of cadmium induces hypothalamic injury through transcriptome profiling alteration and neuronal degeneration in female rats.
Computationally reconstructing cotranscriptional RNA folding from experimental data reveals rearrangement of non-native folding intermediates.
Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research.
Genome-Wide Discovery of Natural Variation in Pre-mRNA Splicing and Prioritizing Causal Alternative Splicing to Salt Stress Response in Rice.
NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway.
HAND1 and BARX1 act as transcriptional and anatomic determinants of malignancy in gastrointestinal stromal tumor.

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Poly-A enrichment or rRNA depletion – how library construction influences RNA sequencing data

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