The human brain has about 100 billion neurons, linked in intricate ways, that the Spanish neuroanatomist Ramón y Cajal compared to “the impenetrable jungles where many investigators have lost themselves.” During the last two decades...
Read More »Single Cell RNA-Seq Reveals the Fundamental Units of the Brain
(a) Experimental workflow started with the isolation, sectioning and microdissection of the primary visual cortex from a transgenic mouse. The tissue samples were converted into a single-cell suspension, single cells were isolated by FACS, poly(A)-RNA from each cell was reverse ...
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