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Tag Archives: CRISPR RNA

A Small RNA isolation and sequencing protocol and its application to assay CRISPR RNA biogenesis

April 3, 2018 Leave a comment 2,355 Views

rna-seq

Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, Stanford University researchers describe a method for isolation and strand...

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Recent RNA-SEQ Pubs

Prepubertal exposure to a high dose of cadmium induces hypothalamic injury through transcriptome profiling alteration and neuronal degeneration in female rats.
Computationally reconstructing cotranscriptional RNA folding from experimental data reveals rearrangement of non-native folding intermediates.
Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research.
Genome-Wide Discovery of Natural Variation in Pre-mRNA Splicing and Prioritizing Causal Alternative Splicing to Salt Stress Response in Rice.
NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway.
HAND1 and BARX1 act as transcriptional and anatomic determinants of malignancy in gastrointestinal stromal tumor.

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • baseclick

    The biotech company baseclick GmbH launched a new and highly efficient method for SARS-CoV-2 mutation analysis

    3 hours ago
  • rna-seq

    New computational tool reliably differentiates between cancer and normal cells from single-cell RNA-sequencing data

    9 hours ago
  • rna-seq

    RNA sequencing of wastewater useful for control of SARS-CoV-2

    10 hours ago
  • rna-seq

    Poly-A enrichment or rRNA depletion – how library construction influences RNA sequencing data

    1 day ago
  • rna-seq

    AIR-seq – metabolic RNA labeling for probing RNA dynamics in bacteria

    1 day ago
  • Featured RNA-Seq Job – Computational Biology Scientist, Cancer Immunology

    2 days ago
  • rna-seq

    Enriching pathogen transcripts from infected samples: a capture based approach to improved host-pathogen RNA-Seq

    2 days ago

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