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Tag Archives: greenfluorescentblog

Sequencing localized RNA in single cells by FISH

March 26, 2014 1 Comment 8,866 Views

rna-seq

from greenfluorescentblog by Gal Haimovich To celebrate the 2-year anniversary of this blog, lets talk about the new Science paper in which the authors claim to performs in situ single cell, single molecule  RNA sequencing. So what’s the big deal? Well, ...

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Recent RNA-SEQ Pubs

Prepubertal exposure to a high dose of cadmium induces hypothalamic injury through transcriptome profiling alteration and neuronal degeneration in female rats.
Computationally reconstructing cotranscriptional RNA folding from experimental data reveals rearrangement of non-native folding intermediates.
Aethionema arabicum genome annotation using PacBio full-length transcripts provides a valuable resource for seed dormancy and Brassicaceae evolution research.
Genome-Wide Discovery of Natural Variation in Pre-mRNA Splicing and Prioritizing Causal Alternative Splicing to Salt Stress Response in Rice.
NOTCH3, a crucial target of miR-491-5p/miR-875-5p, promotes gastric carcinogenesis by upregulating PHLDB2 expression and activating Akt pathway.
HAND1 and BARX1 act as transcriptional and anatomic determinants of malignancy in gastrointestinal stromal tumor.

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • Featured RNA-Seq Job – Computational Biology Scientist, Cancer Immunology

    4 hours ago
  • rna-seq

    Enriching pathogen transcripts from infected samples: a capture based approach to improved host-pathogen RNA-Seq

    5 hours ago
  • rna-seq

    Associate Professor

    14 hours ago
  • rna-seq

    Using single-cell RNA sequencing to disentangle the blood system

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    Arriba – accurate and efficient detection of gene fusions from RNA sequencing data

    3 days ago
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    MSRG – new database of mosquito small RNA transcriptomes

    4 days ago
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    Single-cell RNA sequencing reveals SARS-CoV-2 infection dynamics in the lungs

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