For model species, the 3′ RNA-seq method might more accurately detect differential expression

December 5, 2016 Leave a comment 6,706 Views

One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3′ RNA-seq method, which yields only one sequence per transcript, bypasses ...

Published reference genomes should be re-annotated before use as references for RNA-Seq experiments

May 27, 2016 Leave a comment 6,994 Views

RNA-seq based on short reads generated by next generation sequencing technologies has become the main approach to study differential gene expression. Until now, the main applications of this technique have been to study the variation of gene expression in a ...

RNA-Seq Blog Transcriptome Research & Industry News

Tag Archives: Length bias

For model species, the 3′ RNA-seq method might more accurately detect differential expression

Published reference genomes should be re-annotated before use as references for RNA-Seq experiments

Tailoring the resolution of single-cell RNA sequencing for primary cytotoxic T cells

Upcoming Workshop – Bioinformatics Bootcamp Advanced Topics – Single Cell RNA-Seq

NDRindex – a method for the quality assessment of single-cell RNA-Seq preprocessing data

Study defines small-cell lung cancer subtypes and distinct therapeutic vulnerabilities for each type

Single-cell Multi-Omics: Marrying RNA Seq to protein expression and more

NASA GeneLab RNA-Seq Consensus Pipeline – Standardized Processing of Short-Read RNA-Seq Data

New approach emerges to better classify, treat brain tumors