Tag Archives: Low-Input

A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative non-ribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oligo(dT)-priming of messenger RNAs to exclude ribosomal...

Gene expression analysis using next-generation sequencing (NGS) has advantages in throughput, sensitivity and accuracy over qPCR and microarray methods. However, current limitations with library preparation strategies and access to data analysis often makes RNA-seq unnecessarily laborious and confusing. This live ...

Obtaining high quality RNA from complex biological tissues, such as the brain, is needed for establishing high-fidelity cell-type specific transcriptomes. Although combining genetic labeling techniques with laser capture microdissection (LCM) is generally sufficient, concerns over RNA degradation and limited yields ...

The advent of next generation sequencing has fundamentally changed genomics research. Unfortunately, standard protocols for sequencing the genome and the transcriptome are incompatible. This forces researchers to choose between examining either the DNA or the RNA for a particular sample. ...

Low-input RNA-seq is powerful to represent the gene expression profiles with limited number of cells, especially when single-cell variations are not the aim. However, pre-amplification-based and molecule index-based library construction methods boost bias or require higher throughput. Here researchers at ...

SAN CARLOS, Calif.–(BUSINESS WIRE)–NuGEN Technologies, Inc. today announced the launch of the Ovation® SoLo RNA-Seq System, the company’s latest tool in its suite of sample preparation products supporting research in areas previously hampered by sample input limitations such as single ...

Phil Ewels from NGI presents a summary of a low-input and low RIN-value study performed by NGI Stockholm in order to investigate the effects of lower input amounts and RIN-values for RNA-seq analysis of mRNA transcripts. This study did not ...

Recent advances in RNA-seq methodologies from limiting amounts of mRNA have facilitated the characterization of rare cell-types in various biological systems. So far, however, technical variations in these methods have not been adequately characterized, vis-à-vis sensitivity, starting with reduced levels ...