Tag Archives: microrna

Background Small RNA sequencing (RNA‐seq) of biofluids is challenging due to the relative scarcity of microRNAs (miRNAs), limited sample volumes, and the lack of a gold standard isolation method. Additionally, few comparisons exist for the RNA isolation and sequencing methods ...

MicroRNAs (miRNAs) are small non-coding RNAs that are key players in the regulation of gene expression. In the last decade, with the increasing accessibility of high-throughput sequencing technologies, different methods have been developed to identify miRNAs...

In recent years, the scientific community has become aware that epigenetic mechanisms play a more important role in gene regulatory networks (GRNs) than was hitherto thought, as accumulating..

A necessary pre-processing data analysis step is the removal of adapter sequences from the raw reads. While most adapter trimming tools require adapter sequence as an essential input, adapter information is often incomplete or missing. This can impact quantification of ...

miRNA-Seq uncovers a link between obesity and breast cancer Obesity increases breast cancer (BC) risk in post-menopausal women by mostly unknown molecular mechanisms. In this study, miRNA profiling by RNA-seq was performed in paired benign and malignant biopsies from 12 ...

Microprocessor, composed of DROSHA and its cofactor DGCR8, initiates microRNA (miRNA) biogenesis by processing the primary transcripts of miRNA (pri-miRNAs). Researchers at the Seoul...

Over 100 types of chemical modifications have been identified in protein-coding and noncoding RNAs (ncRNAs). However, the prevalence, regulation, and function of diverse RNA modifications remain largely unknown. Researchers from the South China University of Technology describe how to annotate, visualize, ...

Crohn’s disease (CD), a chronic inflammatory condition of the intestinal tract, has emerged as a global disease, with rates steadily increasing over...

To comprehensively study extracellular small RNAs (sRNA) by sequencing (sRNA-seq), researchers at the Vanderbilt University Medical Center developed a novel pipeline to overcome current limitations in analysis entitled, "Tools for Integrative Genome analysis...

Extracellular vesicles (EVs) are intercellular communicators with key functions in physiological and pathological processes and have recently garnered interest because of their diagnostic and...