Doctors may soon be able to more accurately diagnose concussions by measuring the number of certain molecules in a person’s saliva, according to Penn State College of Medicine researchers. The results of a recent clinical study confirmed that a patient...
Read More »Comparison of RNA isolation and library preparation methods for small RNA sequencing of canine biofluids
Background Small RNA sequencing (RNA‐seq) of biofluids is challenging due to the relative scarcity of microRNAs (miRNAs), limited sample volumes, and the lack of a gold standard isolation method. Additionally, few comparisons exist for the RNA isolation and sequencing methods ...
Read More »BrumiR – a toolkit for de novo discovery of microRNAs from sRNA-seq data
MicroRNAs (miRNAs) are small non-coding RNAs that are key players in the regulation of gene expression. In the last decade, with the increasing accessibility of high-throughput sequencing technologies, different methods have been developed to identify miRNAs...
Read More »Techniques for small non-coding RNA analysis in seeds
In recent years, the scientific community has become aware that epigenetic mechanisms play a more important role in gene regulatory networks (GRNs) than was hitherto thought, as accumulating..
Read More »Accurate adapter information is crucial for reproducibility and reusability in small RNA-Seq studies
A necessary pre-processing data analysis step is the removal of adapter sequences from the raw reads. While most adapter trimming tools require adapter sequence as an essential input, adapter information is often incomplete or missing. This can impact quantification of ...
Read More »miRNA-Seq uncovers a link between obesity and breast cancer
miRNA-Seq uncovers a link between obesity and breast cancer Obesity increases breast cancer (BC) risk in post-menopausal women by mostly unknown molecular mechanisms. In this study, miRNA profiling by RNA-seq was performed in paired benign and malignant biopsies from 12 ...
Read More »RNA-Seq reveals the molecular basis for the single-nucleotide precision of primary microRNA processing
Microprocessor, composed of DROSHA and its cofactor DGCR8, initiates microRNA (miRNA) biogenesis by processing the primary transcripts of miRNA (pri-miRNAs). Researchers at the Seoul...
Read More »RMBase – decoding the atlas of RNA modifications from epitranscriptome sequencing data
Over 100 types of chemical modifications have been identified in protein-coding and noncoding RNAs (ncRNAs). However, the prevalence, regulation, and function of diverse RNA modifications remain largely unknown. Researchers from the South China University of Technology describe how to annotate, visualize, ...
Read More »Crohn’s Disease subtype accurately predicted through miRNA sequencing
Crohn’s disease (CD), a chronic inflammatory condition of the intestinal tract, has emerged as a global disease, with rates steadily increasing over...
Read More »TIGER – bioinformatic analysis of endogenous and exogenous small RNAs
To comprehensively study extracellular small RNAs (sRNA) by sequencing (sRNA-seq), researchers at the Vanderbilt University Medical Center developed a novel pipeline to overcome current limitations in analysis entitled, "Tools for Integrative Genome analysis...
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