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Tag Archives: Pathview

Tutorial – RNA-seq differential expression & pathway analysis with Sailfish, DESeq2, GAGE, and Pathview

December 10, 2015 1 Comment 10,510 Views

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Using single-cell RNA sequencing to disentangle the blood system

    1 day ago
  • rna-seq

    Arriba – accurate and efficient detection of gene fusions from RNA sequencing data

    1 day ago
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    MSRG – new database of mosquito small RNA transcriptomes

    2 days ago
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    Single-cell RNA sequencing reveals SARS-CoV-2 infection dynamics in the lungs

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    Genuity Science wins 2021 BIG Innovation Award for novel approach to analysis of single-cell RNA sequencing data

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    3 days ago
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    Re­sponse to in­fec­tion ther­apy bet­ter un­der­stood thanks to a new sequencing tech­nique

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