Tag Archives: read mapping

Accurate mapping of tRNA reads

rna-seq

Many repetitive DNA elements are transcribed at appreciable expression levels. Mapping the corresponding RNA sequencing reads back to a reference genome is notoriously difficult and error-prone task, however. This is in particular true if chemical modifications introduce systematic mismatches, while ...

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How to count multi-mapping reads?

rna-seq

RNA-Seq is currently used routinely, and it provides accurate information on gene transcription. However, the method cannot accurately estimate duplicated genes expression. Several strategies have been previously used (drop duplicated genes, distribute uniformly the reads, or estimate expression), but all ...

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DART – novel RNA-seq de novo mapping algorithm

rna-seq

In recent years, the massively-parallel cDNA sequencing (RNA-Seq) technologies have become a powerful tool to provide high resolution measurement of expression and high sensitivity in detecting low abundance transcripts. However, RNA-seq data requires a huge amount of computational efforts. The ...

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(Post) Graduate Course ‘The Power of RNA-Seq’

Date: February 10th-12th, 2016 Location: Room PC95 (first floor), RADIX building (107), Wageningen Campus, Droevendaalsesteeg 1, Wageningen, the Netherlands Directions: directions (check also Map Wageningen UR Campus) Language: English Group size: maximum of 35 participants Credits: 0.8 ECTS Registration and ...

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