Tag Archives: scRNASeq

scPLS – Controlling for confounding effects in single cell RNA sequencing studies using both control and target genes

rna-seq

Single cell RNA sequencing (scRNAseq) technique is becoming increasingly popular for unbiased and high-resolutional transcriptome analysis of heterogeneous cell populations. Despite its many advantages, scRNAseq, like any other genomic sequencing technique, is susceptible to the influence of confounding effects. Controlling ...

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MAST – a flexible statistical framework for assessing transcriptional changes and characterizing heterogeneity in single-cell RNA sequencing data

rna-seq

Single-cell transcriptomics reveals gene expression heterogeneity but suffers from stochastic dropout and characteristic bimodal expression distributions in which expression is either strongly non-zero or non-detectable. Researchers at the Fred Hutchinson Cancer Research Center propose a two-part, generalized linear model for ...

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