Tag Archives: short read

Recent research into structural variants (SVs) has established their importance to medicine and molecular biology, elucidating their role in various diseases, regulation of gene expression, ethnic...

Some sequencing applications, such as the detection of single nucleotide polymorphisms, can be managed with short-read technology. Other applications, such as the detection of structural variants, may demand long-read technology, and some applications, such as the assembly of a new ...

Short-read sequencing has enabled the de novo assembly of several individual human genomes, but with inherent limitations in characterizing repeat elements. Now, an international team led by researchers at USC have sequenced a Chinese individual HX1 by single-molecule real-time (SMRT) ...

The amount of sequence information in public repositories is growing at a rapid rate. Although these data are likely to contain clinically important information that has not yet been uncovered, our ability to effectively mine these repositories is limited. Researchers ...

Next-generation sequencing of cellular RNA (RNA-seq) is rapidly becoming the cornerstone of transcriptomic analysis. However, sequencing errors in the already short RNA-seq reads complicate bioinformatics analyses, in particular alignment and assembly. Error correction methods have been highly effective for whole-genome ...

This is the material for two workshops given at MSU by Titus Brown and Matt Scholz. The first workshop (Dec 4/5) was on model organism mRNAseq, and the second workshop (Dec 10/11) will be on semi-model organism mRNAseq. Both workshops ...

from Genetic Engineering News by Jonas Korlach Long-Read Sequencing Can Offer the Most Comprehensive View Yet of Gene Activity Not too long ago, the life sciences community was still debating whether sequencers would ever overtake microarrays as the preferred means ...