Single-cell RNA sequencing (RNA-seq) provides a powerful tool for the interrogation of gene expression within individual cells. By revealing cell-specific information not accessible through next-generation sequencing of heterogeneous cell populations, single-cell transcriptomics offers the potential to answer important questions about rare, disease-associated cells—such as circulating tumor cells—or about distinct cell types and lineages in healthy tissues. Despite its strong potential, single-cell transcriptomics faces several challenges, including working with the minute amounts of RNA typically present in individual cells, generally in the low-picogram range, as well as the potential loss of molecular complexity, particularly the loss of low-abundance transcripts when preparing sequencing libraries. In this webinar we’ll explore the exciting new field of single-cell RNA sequencing and discover some of the methods scientists are applying to unlock the secrets hidden in the transcriptomes of normal and diseased cells.
During the webinar, our speakers will:
- Outline the advantages and challenges of working with single cells
- Describe cutting-edge methodologies for performing single-cell RNA-seq
- Illustrate applications for single-cell RNA-seq that answer previously intractable questions
- Answer your questions during the live broadcast.